Revisão da leucina aminopeptidase no trabalho de parto pré-termo e na pré-eclampsia / Revision of the leucine aminopeptidase in preterm labor and in preeclampsia

Realizou-se um levantamento bibliográfico, com artigos de revisão, analisando e discutindo os trabalhos publicados sobre os efeitos da leucina aminopeptidase e aminopeptidase A no trabalho de parto pré-termo e na pré-eclampsia. A proposta deste trabalho sobre o tema é que grande parte das questões de saúde materna parece pueril, principalmente quanto ao atendimento voltado para os cuidados maternos, no qual, a cada 20minutos, morre uma mulher em decorrência de parto, no mundo todo. Por isso, tais doenças poderão receber mais atenção do que outras. Esse fato fez com que houvesse certa preocupação com o índice de natalidade e morbidade materna, bem como morbidade e mortalidade perinatal. Portanto, abordou-se sobre sua biologia geral, fisiologia de reprodução, síntese de evolução genética, habitação, alimentação, manejo, dor e eutanásia, técnicas de riscos desenvolvidos na experimentação animal, estudos de experimentos farmacológicos e toxicológicos observados dentro dos artigos de revisão. Embora tendências atuais preconizem a utilização de métodos alternativos (estudo in vitro), os modelos animais, como as ratas, apresentam como principal vantagem o fornecimento de informações sobre o organismo como um todo, fato que não é obtido com outros métodos, o que ainda possibilita o seu emprego em pesquisas científicas.

We have carried out a literature review, with review articles, analyzing and discussing the works that have already been published on the effects of leucine aminopeptidade and aminopeptidase A in pre-term labor and preeclampsia. The proposal of this work on the subject is that most of the issues of maternal health seems childish, especially for service oriented maternity care, where, every 20 minutes, a woman dies due to childbirth, worldwide. Therefore, such diseases may receive more attention than others. This led to worry about the birth rate and maternal morbidity and perinatal morbidity and mortality. Therefore, it was addressed their general biology, physiology of reproduction, synthesis of evolution, genetics, housing, feeding, pain and euthanasia techniques developed for animal experimentation risks, studies of pharmacological and toxicological experiments observed within the review articles. Although current trends have preconized the use of alternative methods (in vitro study), animal models, such as rats, have as main advantage the provision of information on the organism as a whole, a fact that is not achieved with other methods, which also allows its use in scientific research.

Evaluation of leucine aminopeptidase and superoxide dismutase as markers in nasopharyngeal carcinoma

This study was designed to define changes of leucine aminopeptidase [LAP] and total superoxide dismutase [SOD] levels in nasopharyngeal carcinoma [NPC] patients after radiotherapy as a trial to correlate these parameters with disease activity and response to treatment. The study comprised 20 patients with NPC and 20 control subjects of those attending blood bank for blood donation. All patients underwent detailed history taking, full ENT clinical examination, and general examination for draining lymph nodes. Lesions characters and TNM staging were determined. All study participants gave blood samples for estimation of total blood SOD and serum LAP [pre-treatment] and were re-estimated 2 and 4 months after radiotherapy. There was a significant [P<0.05] increase in patients age and number of males [18:2] with significant increased incidence in smokers [16:4]. Eleven patients had stage I, 4 patients had stage II and 5 had stage III lesions. All patients with stage II and III lesions and 7 with stage I were smokers. Pre-treatment levels of LAP showed a significant [P<0.05] increase compared both to control, 2-m and 4-m levels with progressive steady decease with treatment. The levels detected 4-m after treatment showed a non-significant difference compared to control levels. The pretreatment SOD levels showed a significant [P<0.05] increase compared to both control and 4-m levels. Also, the SOD levels detected 2-m after treatment showed a significant [P<0.05] increase compared both to control and 4-m levels. There was a positive significant correlation between pretreatment levels of LAP, [r=0.85 1, P<0.001] and SOD, [r=0.643, P=0.002] and progress of tumor growth manifested as stage. We can conclude that pretreatment determination of total SOD and serum LAP correlate with severity and aggressiveness of NPC and could be used as an aid for staging. Furthermore, serum LAP correlates with response of NPC to radiotherapy and could be used to follow-up patients and as a prognostic marker

Serum leucine amino peptidase levels as a marker in testicular tumours.

Serum levels of leucine aminopeptidase (LAP) were studied in 60 patients with testicular tumours and 26 normal individuals, and were found to be significantly elevated in patients with seminomas, nonseminomas and benign tumours of the testis. After treatment the LAP levels decreased in patients with seminomas, nonseminomas and mixed cell carcinomas indicating response to therapy. The quantitative method used for estimation of LAP, which is simple, quick, accurate and cost-effective, could be used more effectively as a tumour marker in testicular malignancies.

Serum leucine aminopeptidase estimation: a sensitive prognostic indicator of invasiveness in breast carcinoma.

Serum Leucine Aminopeptidase (LAP) was studied in 25 patients of breast tumors, and compared with 50 age matched controls. Clinical staging was done in 13 cases of breast carcinoma. Serum LAP was increased but not significantly in stage I (P less than 0.1), while in stage II & III, significant increase was observed (P less than 0.05), but a sharp peak was noted in stage IV with metastasis in liver (P less than 0.001). Serum LAP values were correlated with histological sub-types of breast carcinoma. Highest serum LAP values were observed in filtrating duct carcinoma with productive fibrosis (P less than 0.01), followed by medullary carcinoma and the enzyme values were slightly decreased in intraductal carcinomas.

Significance of serum leucine aminopeptidase in tumours of female genital tract.

Serum leucine aminopeptidase (LAP) was estimated in 57 female patients with tumours of the genital tract and compared with 50 age-matched controls. Serum LAP was increased in leiomyomas (p less than 0.01) but significant rise was seen in leiomyosarcomas and endometrial carcinoma of the uterus (p less than 0.001). No increase in serum LAP level, above mean control value was observed in carcinoma of cervix. LAP levels were also increased in cystadenomas of the ovary (p less than 0.01) and highly significant increase, noted in cystadenocarcinomas and dysgerminoma stage III (p less than 0.001). However, no increase of enzyme activity was observed in poorly differentiated papillary carcinoma of the ovary. The serum enzyme level was higher in cystadenocarcinomas as compared to solid ovarian carcinomas in the same clinical stage.

Enzimas hepáticas: níveis séricos em crianças normais / Hepatic enzymes: serum levels in normal children

Descrevem-se as características químicas e clínicas de algumas enzimas utilizadas como indicadores de doença hepática. Säo determinadas as atividades séricas das aspartato-aminotransferase, alanina-aminotransferase, gama-glutamil-transferase e leucina-aminopeptidase no soro de 225 crianças normais com idades de 0 a 12 anos. Os resultados evidenciam diferenças significativas em relaçäo aos níveis de adultos, geralmente utilizados como referência. Estas diferenças foram consideradas como resultado da imaturidade celular e/ou estado hipermetabólico

La leucinoaminopeptidasa: su determinación en ninos y adultos noramles / Leucine aminopeptidase: its determination in children and in normal adults

Tomando como referencia los valores de adultos normales, se determina la actividad leucinoaminopeptidosa en suero de embarazadas, puérperas, sangre de cordón, recién nacidos, lactantes y niños normales, de distintas edades, reuniendo en total 59 observaciones. Se analiza el papel de la placenta, como fuente originaria de actividad LAP en la gravidez y también se bosquejan posibles mecanismos para explicar la elevación de la actividad enzimática en sangre de cordón, recién nacidos, y otras edades.